... be slowest. * METHOD MATERIALS USED: Electric Kettle, 100ml Beaker, 10 Boiling tubes, 2 Boiling tube racks, Milk, Sodium Carbonate, Phenolphthalein, Spirit Thermometer, 5% Lipase. PROCEDURE: STEP 1: We mixed 5cm³ of milk with 7cm³ of Sodium Carbonate and added 6 drops of phenolphthalein into each of 5 boiling tubes. STEP 2: We next prepared 1cm³ of Lipase into the remaining 5 boiling tubes. STEP 3: Boiling water from the kettle was then added to the beaker and with cold water the temperature was lowered to 80°C. STEP 4: Into this beaker was then placed a boiling tube containing the milk mixture and a boiling tube containing the lipase, and were left to stand for 5 minutes. STEP 5: After 5 minutes of allowing the two mixtures to heat up the lipase was poured into the milk mixture, gently shaken and then placed back into the beaker. STEP 6: We then time how long it took for the pink colour change to a white mixture indicating the fat had been hydrolysed. STEP 7: The ...

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