... in principle this technique could be applied to any plant tissue. Apparatus * Boiling tubes x6 * Boiling tube rack * Wax pencil * Cork borer * Razor blade or sharp knife * White tile * Root/tuber * Filter papers * Forceps * Balance to 0.001g * Distilled water * 1.0 mol sucrose solution * 2x 25cm3 measuring cylinders * Stop clock Procedure 1. Label 6 boiling tubes "DW" (distilled water), 0.2, 0.4, 0.6, 0.8 and 1.0 mol dm3. Place 20ml of distilled water in the first tube and the appropriate amounts of sucrose solution and water in each of the other tubes to make up the correct concentrations. 2. Using a cork borer and a razor blade or sharp knife, prepare 6 root/potato cylinders, each about 10mm diameter and 50mm long. Place each on a separate piece of filter paper on which you have labelled with the figures 0, 0.2, 0.4, 0.6, 0.8 and 1.0 respectively. 3. Take the cylinders and the boiling tube rack to a balance. ...

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