... increase the rate of reactions without being used up in the reaction themselves. When we analyse rates of reaction we can see that the amount of oxygen made increases as we add higher concentration. However, eventually the rate of reaction will reach an optimum level. The temperature and PH will also affect the enzyme. 37 degrees is the optimum level enzymes work as this is body temperature. After this the enzymes start to denature, this is a permanent change and therefore it is irreversible. The same applies to PH. This I will investigate, but the main reason of my experiment is to focus on the concentration of substrate. The substrate we are using is Hydrogen Peroxide and the enzyme is catalyse (potato). The catalyse breaks down Hydrogen Peroxide into water and Oxygen. 2HO2--> 2h2O + O2. I will be using different concentrations of Hydrogen Peroxide to see the affect it has ...

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