... with the Human eye .Two factors are known to be vital in providing the examiner with accurate results of the specimen being examined, magnification and resolution. Magnification is the number of times larger an image is then the actual specimen. Resolution is the degree of detail, which can be seen. Shorter wavelengths give the best resolution. In general it can be said that the greater the magnification the grater the resolution. Early Microscopists saw quite distorted images due to the low quality of the glasses and imperfect shape of their lenses. Dramatic progress in the development of microscopes took place during the 19th century. In order for light microscopes to achieve better resolution, three basic problems had to be over come. (1) The first problem was the unequal bending of different colors of light that occur in lenses. (2) is the unequal bending of light that hits different parts of ...

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