... the fine focusing knob, the image was focused until the object was sharp. Method - Preparing a temporary slide 1) An onion bulb was cut through vertically and one of the leaves of the bulb was separated off. 2) The inner surface was carefully peeled off with a pair of fine forceps. 3) A small piece of the epidermis (5mm x 5mm) was cut. 4) A pipette was used to carefully place a drop of water on a clean microscope slide. 5) The epidermis was carefully placed in the drop of water. A mounted needle was used to flatten it. 6) A cover slip was placed on top of the epidermis, to make sure that the tissue was kept flat. 7) A drop of iodine solution was placed on one side of the cover slip. 8) The cut edge of a piece of filter paper was placed to the other side of the cover slip to draw small amounts ...

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