... and appear fuzzy).The two types of microscopes are electron and light microscopes. Principles and Limitations of light microscopy Light microscopes function by focussing a beam of light on the object to be examined.The beam passes through a series of lenses after passing through the specimen.The condenser lens focuses the light onto the specimen for maximum illumination.The lower objective lens magnifies the object ,and the image from this lens is further enlarged when viewed through the upper eyepiece lens.The beam from the light source directly enters the observer's eye.It has a few limitations.Material to be examined by light microscopy must be sufficiently transparent and thin enough for light to pass through .They are cheap,portable and easy to handle.However they cannot resolve anything that is less than 0.2 micro metre apart This limit is due to the wavelength of light.For this reason they can't examine minute organisms like viruses nor can they readily ...

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