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To investigate the effect of temperature on the enzyme Catalase.
... be very careful in our actions around the lab.
Catalase is an enzyme found in all living cells. It makes Hydrogen Peroxide decompose into water and oxygen. We will measure the amount of Oxygen released from the Hydrogen Peroxide. In order ...
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To investigate the effect of temperature on the rate of reaction on trypsin enzymes.
... results from my preliminary experiment have shown me that the acclimatisation time should be 4 minutes, and that an end point of 10 minutes is enough time for us to be able to tell that no reaction will take place. ...
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To investigate the effect of temperature on the time taken for pepsin to digest egg albumen.
... of cells in the body.
Enzymes can become denatured in extreme heat conditions or in unfavourable pH conditions. The bonds in enzymes are weak, thus when the above stated conditions are reached the bonds are broken, deforming the enzyme and its ...
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To investigate the effect of the substrate concentration, on the rate that it converts hydrogen peroxide to water and oxygen, catalysed by the enzyme peroxidase.
... syringe) and take readings every 10seconds for 90 seconds and place in a table as shown below this method.
5. Now repeat steps 2, 3 and 4 for the different concentration as shown in the table above. Take the results and ...
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To investigate the effect of Trypsin using a photographic film
... alkali pH.
Secondly the temperature. Every enzyme has a temperature range of optimum activity. Modification of the lock and key hypothesis assumes that the active site has a certain amount of elasticity whereby the active site can expand or contract ...
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To investigate the effects oftemperature on catalyse action?
... The formula for this experiment is:
Hydrogen peroxide --> oxygen + water
2H2 O2 --> O2 + 2H2O
This information is found from www.s-cool.co.uk
Experiment
This is how my apparatus will look like:
Apparatus
The apparatus which I will be using for this experiment ...
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To investigate the factors affecting the activity of the enzyme Amylase, I will adapt a pilot experiment, which investigates if the enzyme amylase breaks down starch.
... down starch.
The pilot experiment was:
* Pour amylase solution into a test tube to a depth of 2cm.
* Half fill another test tube with a 4% starch solution.
* With a pipette place a drop of iodine into each dimple in a ...
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To investigate the factors of the enzyme hydrogen peroxidase (catalase)
... can be repeated as often as 100,000 times per second. Enzymes are very specific in relation to the substrates with which they work, and are normally only effective for one reaction or a group of closely related reactions. They function ...
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To investigate the factors that affect the time taken for the digestion of casein in milk by trypsin, and to see what factors will make the reaction go faster or slower.
... to one chemical reaction. An ordinary catalyst may be used for several different chemical reactions, but an enzyme usually only works for one specific reaction. Each particular enzyme has a unique, 3D shape shared by all its molecules. Within this ...
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To investigate the optimum concentration of an enzyme in an enzyme controlled reaction
... predict the optimum concentration because this is what I am trying to find.
My graph can be divided into two sections the first is the curved region. This occurs because each enzyme that is added joins up with one substrate ...
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To investigate the possible reasons for fruits, such as tomatoes, becoming softer and squashier as they ripen.
... In industry and at home, Pectin is well known for its gelling, thickening and stabilizing properties. Today, it is used in such diverse applications as yoghurt, confectionery and acid milk drinks. It has the image of a natural product and ...
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To investigate the rate at which hydrogen peroxide is broken down by the enzyme Catalase (Celery Extract). When the hydrogen peroxide decomposes, oxygen
... reaction, but remains unchanged at the end of the reaction. Virtually every metabolic reaction that takes place within a living organism is catalysed by an enzyme. Enzymes are made of proteins, in their globular structure, one or more polypeptide chains ...
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to investigate the volume of amino acid produced from broken down proteins
... of hydrogen peroxide. One enzyme can be used many times over. This is because a catalyst is a chemical substance that speeds up a reaction but it does not get used up during the reaction.
Types of enzymes
Enzymes are made inside ...
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To plan an investigation into the effect of substrate concentration on the activity of the enzyme catalase.
... altering the concentration
Graduated Biuret - To measure the volume of oxygen collected
Water Tank - Holds water and upside down measuring cylinder for viewing oxygen bubbles
Conical flask - Where the yeast solution and substrate shall be mixed
A cork with one ...
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To see how much oxygen is produced from celery when it is put into hydrogen peroxide and also to see how the different concentration affects the rate of reaction.
... all the enzymes, there is an active site where the substrate molecules are converted to the enzyme molecule and from there they are converted to product molecules.
All enzymes are shaped in their own way so then they would be able ...
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To see how much oxygen is produced from celery when it is put into hydrogen peroxide and also to see how the different concentration affects the rate of reaction.
... all the enzymes, there is an active site where the substrate molecules are converted to the enzyme molecule and from there they are converted to product molecules.
All enzymes are shaped in their own way so then they would be able ...
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To see how well the digestion enzyme pepsin will break down protein when subjected to various temperatures.
... The stomach has three mechanical tasks to do. Firstly Muscles in the stomach relax and allow large amounts of food to be stored, secondly the stomach releases the protease enzyme (pepsin) which breaks down proteins into smaller pieces called peptides. ...
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To see if Enzymes Catalysts are affected by temperature.
... the part of the enzyme that allows it to be a catalyst. As below:
Anabolic reaction.
Molecules
of substrate
Enzyme
Active site
The enzyme Molecule is now free to bind with more molecules of substrate. Each enzyme Molecule may be used many thousands of ...
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To see if temperature makes a difference to the time taken for starch to be broken up and turned into glucose by amylase.
... again
Working best at a neutral pH value and at the bodies temperature, a rise above 50 degrees celsius changes the shape of an enzyme and they can no longer work.
In my experiment Amylase is the enzyme, which ...
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To see what effect temperature has on the speed of enzyme activity.
... won't be very accurate, as we will only be able to give the time to the nearest minute.
Fair Testing:
In this experiment there is only one variable, and that is the temperature of the water in the glass beaker. Everything else ...
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To show how the change in temperature affects the speed that an enzyme works
... example being the human stomach, which is kept at a constant temperature of around 37°C, but if the environment in which the enzyme is working becomes too hot then the enzyme will start to denature; which is where it will ...
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To study the relationship between temperature and the speed at which amylase works.
... the amylase to work effectively.
From my results I can tell that my original hypothesis; 'I predict that as the temperature increases amylase will break starch down into glucose faster,' is partly correct, as the temperature rises from 31°C -34°C ...
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To understand what enzymes are and what they do.
... can digest the gelatine by the protease enzyme pepsin leaving silver salts behind. As they are no longer supported by the gelatine they will fall to the bottom of the test tube and the film that was in contact with ...
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To what extent are enzymes used today in industry, medicineand the food industry
... different distillations, the enzymes can make alcohol of up to 20% proof so it is usually used to make beer and wine as the individual tastes of these products make the market extensive and wide in variety. The disadvantage of ...
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To what extent do you agree with the view that "Parliament is a policy influencing body, whereas congress is a policy making body".
... substrate specific. The active site of each different enzyme has its own particular tertiary structure, so only a substrate with a complementary shape will fit; we say it has a highly specific shape. In order to work, an enzyme must ...