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To investigate a certain factor that will affect the rate of breakdown of the protein gelatine using trypsin.
... a black protean gelatine coating and trypsin breaks down proteins so therefore will break down the coating and the film will just become a clear strip of plastic. Trypsin usually works at pH8 or pH9 (alkali conditions) and at human ...
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To Investigate an Enzymes Reaction.
... each time. I will use six different concentrations of the substrate and go down in the amount of percentage used.
E.g. 100% - H2O2, 80% - H2O2 + 20% - H2O, 60% - H2O2 + 40% - H2O etc.
I will see what ...
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To investigate and compare the activity of immobilised enzymes against enzymes free in solution at a range of different concentrations.
... The human body temperature is 37C, and without catalysts the reactions would be too slow to sustain life.
Since there are thousands of different reactions happening in cells, and each requires a specific enzyme, enzymes must be made of ...
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To investigate changes in the rate of reaction under different temperatures using yeast.
... they are used in biological detergents and washing powders. They break down foods, blood and other biological substances. These biological detergents are more effected than ordinary because they contain enzymes as well as soaps and detergents. The enzymes for these ...
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To investigate factors that affects the rate of breakdown of the protein gelatine by trypsin.
... Cylinder
Controlled.
Amount of Gelatine.
cm.
Ruler
Prediction:
I am going to change the temperature that the trypsin works at. The time taken for the trypsin to digest the gelatine will change because of this.
I predict that the temperature that the trypsin works at ...
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To investigate how different factors affect the rate of breakdown of sucrose by the enzyme sucrase.
... investiagtion I will use the enzyme sucrase to catalyse the substrate sucrose into glucose and fructose molecules.
Affecting Factors
There are several factors which affect the efficiency of enzymes. These are temperature, pH values and concentration of the enzyme and substrate.
pH Value
There ...
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To investigate how temperature affects the concentration of vitamin C in orange juice (specifically 'Just Juice').
... has turned a purply-brown colour). I predict that when I plot a graph of temperature against volume of DCPIP used, I will get a straight-line, indicating direct correlation between the two.
Method: In order that we obtained the most accurate ...
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To investigate how temperature affects the enzyme amylase.
... will be denatured and will no longer work. Only few cells can accept temperatures high than approximately 45c. Enzymes work by having an active site substrate molecules, which will join with the enzyme and a reaction will take place. The ...
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To Investigate how temperature affects the rate at which catalase catalyses hydrogen peroxide.
... the thermometer until the right temperature has been reached
5) Keep the temperature steady for 2 minutes by removing the heat (if it drops dramatically, heat again) to allow the test tubes to remain at a constant heat
6) Take out the ...
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To investigate how temperature affects the rate at which the enzyme Catalase breaks down hydrogen peroxide into oxygen and water
... my hypothesis.
First of all I will use the 'lock and key' theory.
All enzymes are specific and there is one enzyme per function. The enzyme that I am investigating is called Catalase and its function is that it can only break ...
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To investigate how temperature affects the rate of reaction of the enzyme catalase on its substrate hydrogen peroxide
... the enzyme it is specified to. This can be explained as a lock and key hypothesis, where the lock and key are specific to each other, only, that there are many of the same kinds of lock and key when ...
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To investigate how the change in temperature affects the performance of the enzyme catalase in the breakdown of hydrogen peroxide (H2O2) into water and oxygen.
... Enzymes are also sensitive to pH, different enzymes have different pH optimums which are determined by their surroundings e.g. pepsin found in the stomach has an optimum of around pH 2 as the stomach is a very acidic part of ...
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To Investigate How the Permeability of the Membrane Changes With Temperature.
... present abundantly in all living cells, and must play an important part in the metabolism of the cell.
For example, the plasma membrane allows the movement of several substances into and out of the cell.
The following diagram shows the substances that ...
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To investigate how the rate of reaction of amylase on starch is affected by the concentration of the substrate.
... should be cleaned away in an adequate way. This will prevent accidents in lab rooms.
Method> Place all needed equipment as shown in the diagram> Make a starch solution which consists of 4 ml of starch and 6 ml of water ...
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To investigate one of the factors that affects the permeability of cell membranes.
... factors that I have found affect the permeability of cell membranes, must somehow destroy part of the cell membrane to allow the pigment molecules to get through and make the surrounding water turn red.
(Sources of information:
www.fed.cuhk.edu.hk/~johnson/tas/investigation/membrane_teacher.htm
C.A.D Discussion ...
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To investigate the affect of temperature on the enzyme trypsin.
... splitting of peptide bonds on the carboxyl side of lysine and arginine residues. Therefore the exclusion of trypsin will reduce dramatically the activity of other proteases. It works best at a slightly alkaline pH (pH 7.6-8). This pH is created ...
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To investigate the amount of O2 released at different of concentration of enzyme catalyse in (Yeast).
... are specific, this means that each enzyme catalyses a certain chemical reaction.
An enzyme has an active site, which has a unique shape into which only a substrate of same unique shape can be fit. When this substrate fits into ...
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To investigate the changes in volume when glucose and yeast are mixed together, and the heat of the solution is changed.
... certain conditions. It can be denatured in high phs and high temperatures. The bonds, which hold the enzyme together, are fairly weak, so they can be easily broken. Once the bonds of the enzyme break, the enzymes active site begins ...
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To investigate the course of an enzyme controlled reaction
... be controlled by using syringes that are accurate to the measurements being used i.e. the smallest syringe for the measurement. For example, I will be measuring 5cm³ of diastase so the most accurate syringe I could use is a 5cm³ ...
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To Investigate the Course of an Enzyme controlled Reaction.
... able to obtain a fairly strong conclusion. Finally, I will evaluate my experiment and results commenting on any errors observed, and what improvements can be made to make the experiment more accurate.
Prediction
I predict that as the concentration decreases ...
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To investigate the effect of a change in temperature on the action of enzymes during starch digestion
... an optimum temperature at which it works best at.
Enzymes are thought to work according to lock and key theory, as enzyme has to fit exactly to substrate.
This idea can also be linked to the collision theory, as the enzyme weakens ...
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To investigate the effect of cell sizes on diffusion by modelling the process of agar blocks and dilute Hydrochloric acid.
... blocks into cubes of 1cm3, 2cm3 and 3cm3 on a white tile. The same shape blocks (cubes) must be used so that it is a fair test. Accurately measuring and cutting them with the metric ruler and knife. The same ...
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To investigate the effect of cellulase on fruit juice extaction.
... paper over)
A hypothesis for this experiment would predict a significant increase in fruit juice extraction using cellulase. Since the hypothesis predicts a change in the dependent variable as the independent variable changes, the appropriate graphical treatment of the data was ...
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To investigate the effect of changing concentration of hydrogen peroxide (H2O2) on the enzyme catalase
... released the most oxygen. We also found that the best amount of yeast to use is 0.5g
Planning
The aim of this investigation is to find out the rate of reaction of catalase on H2O2. The variable I am going to ...
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To Investigate the effect of changing temperature on the action of the enzyme catalyse, by measuring the volume of gas produced in 20 seconds
... the temperature to 30, 35 and 40?c, this is where I will see the greatest reaction. I predict this because enzymes are designed to work best at the internal body temperatures of the animals they belong to. For a mammal, ...