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Essays in Molecules & Cells category
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This piece of coursework is about the enzyme catalase. Enzymes are used all over the body for different jobs such as the breaking down of food. I did an experiment to find out at what temperature did the most activity happen.
... a fair test or not. If so, how did I make it a fair test? How did I ensure that all the equipment was suitable to give each set of results an equal chance?> What safety procedures did I ...
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To carry out an investigation into the denaturing of immobilised lactase enzymes in relation to thermodynamics.
... sweetener, and can be found in small amounts in infant and dietary foods and coffee whiteners. Lactose is very inexpensive due to its existence in great amounts. The splitting of lactose can be brought about with the use of the enzyme, ...
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To determine if the rate of reaction of free enzymes is higher that the rate of reaction in immobilised enzymes in different pH concentrations of solution.
... optimum level, then the enzyme will denature. If the temperature is too high, the bonds of the 3D shape will break, and thus the enzyme will denature. Every enzyme has an optimum pH, (the pH at which the enzyme will ...
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To determine the effect of Detergent on the Permeability of Cell Membranes.
... is to act as transport proteins, which are hydrophilic channels, which only allow certain ions or molecule through, with each channel being specific to a certain one. The cell membrane is said to be partially permeable and acts as a ...
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To determine the optimum pH for two different proteolytic enzymes.
... most active. The optimum pH of each enzyme is represented by the crest of the graph of enzymatic reaction against pH of buffer solution. Buffer solution is a solution which resists the change in its pH value by adding acid, ...
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To examine the effect of temperature on the enzyme catalase.
... that other molecules fit into the active site. This is known as either competitive or non competitive inhibition * What is Catalase specifically Catalase is an enzyme found in most creatures. It converts naturally produced hydrogen peroxide into water ...
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To find out how different concentrations of sucrose solution affect the incipient plasmolysis of different root vegetables until the isotonic point has been reached.
... Pure water contains the highest water potential which is zero kilopascals 0(?) All solutions have a lower water potential then pure water hence there water potentials are always negative, the more solute molecules present the lower more negative the water ...
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To find out how enzyme concentration affects the rate of an enzyme controlled reaction
... glucose molecules. Aim To find out how enzyme concentration affects the rate of an enzyme controlled reaction Hypothesis I believe that the effect of increasing the concentration of the enzyme will increase the speed with which the enzyme-controlled reaction takes place. This prediction is correct ...
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To find out how is enzymes affect by different type of temperature and see the changes in the colour of the mixture of iodine, starch and amylase.
... and will therefore not react so quickly. Eventually the shape of the amylase molecule changes too much and the starch molecule will not be able to fit on at all. This means that the amylase will not be able to ...
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To find out how temperature affects amylase.
... will supply the molecules with more energy to react, and then it will slow down and the rate of reaction will be a lot longer. Apparatus: * Bunsen burner * Tripod * Gauze * Beaker * 2 test tubes * 2 spotting tiles * ...
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To find out how temperature affects the breakdown of starch by the enzyme amylase.
... insure that the water is the same temperature through out each experiment and that the temperature is accurate. If my water is too cold I can add hot water and if it is too hot I can add cold water. ...
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To find out how temperature affects the rate of anaerobic respiration by yeast
... solution-glucose concentration ____) * Temperature - as I am testing this variable I will need to vary it but I will have to keep it constant throughout each individual test. I will do this by placing a thermometer in the water ...
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To find out how the rate of reaction of yeast can be increased
... called zymase. A chemical reaction always involves one substance being changed into another. The substance that is present at the beginning of the reaction is called the substrate. The substrate that is made by the reaction is called the product. ...
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To find out if temperature affects the amount of juice the enzyme pectinase releases from an apple.
... and the substrate products are released separately. Because the substrate molecules are different shapes they will only fit into certain enzymes. When the enzyme reaches a temperature too high it will change shape, therefore the substrate molecule would not fit. ...
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To find out if temperature affects the amount of juice the enzyme pectinase releases from an apple.
... and the substrate products are released separately. Because the substrate molecules are different shapes they will only fit into certain enzymes. When the enzyme reaches a temperature too high it will change shape, therefore the substrate molecule would not fit. ...
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To find out if temperature directly affects the activity of protease enzyme
... of 20oc (I will repeat this process for the further temperatures (listed in results table)). When I was sure that my evidence would be valid I placed each test tube into the beaker, making sure that each film strip was ...
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To find out if the strength of a solution affects the amount of water passing into and out of cells in potato.
... the cell, this decreases the pressure of the vacuole on the cytoplasm until the cytoplasm comes away from the cell wall. This makes the cell limp and is known as Plasmolysed. The following diagram further explains this theory: Also if the ...
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To find out the effect of increasing the concentration of hydrogen peroxide on the rate of catalytic reaction using catalyse.
... substance will not work on a different one. The substance on which an enzyme acts is called its substrate. The diagram below shows how the shape of an enzyme could decide what substrate it combines with. The enzyme has a ...
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To find out the optimum temperature for the enzyme trypsin.
... of marvel/milk in my experiment. I will put both of the liquids into two different test tubes and them put them into a beaker. I will fill the beaker with water and then heat the trypsin and the milk up. ...
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To find out what a change in concentration of concentration of enzymes does to the length of time it takes to make the substrate into the product. I will use starch as the substrate, and amylase as the enzyme.
... highest. I also predict that the graph at the end will be a straight line of concentration on the x-axis and time on the y-axis. I think that the concentration of enzymes will be directly proportionate to the time taken, ...
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To find out what factors affect the rate of reaction between rennin and milk.
... RNA. Enzymes have catalytic properties; in other words, they alter the rate of reaction without themselves undergoing a permanent change. Most chemical reactions require an initial input of energy, called activation energy, to enable them to occur. Enzymes reduce the ...
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To find the effect of temperature on enzymes, using a potato as a catalyst
... are large proteins that speed up chemical reactions. In their globular structure, one or more polypeptide chains twist and fold, bringing together a small number of amino acids to form the active site, or the location on the enzyme where ...
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To find the effect of the enzyme concentration on the reaction between Catalase and hydrogen peroxide.
... be made up of different configurations of polypeptide chains. There are secondary, tertiary and quaternary structures (i.e. haemoglobin) protein molecules which increase respectively in complexity. Proteins contain an 'R-group' which is specific to each amino acid, this part of the ...
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To find the optimum temperature that the enzyme catalyse works at.
... temperatures. I will then have to have a reaction that can be controlled to measure the efficiency and speed of the reaction. Catalase reacts violently with hydrogen peroxide, therefore to get a reaction I shall put the liver containing catalase ...
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To Investigate (By Experimentaion) the Effect of Substrate Concentrations On the Rate of the Decomposition of Hydrogen Peroxide When Catalyzed By the Enzyme Catalase.
... of the fastest acting enzymes known. It catalyses the decomposition of hydrogen peroxide, liberating oxygen gas as effervescence, each molecule of the globular protein decomposing 40,000 molecules of hydrogen peroxide per second at zero degrees Celsius and capable of producing ...