... an optimum temperature at which it works best at. Enzymes are thought to work according to lock and key theory, as enzyme has to fit exactly to substrate. This idea can also be linked to the collision theory, as the enzyme weakens the bond at the active site, the activation energy needed on average to break the bond is lowered, and the bond is broken by a water molecule. We also know that if the temperature is increased the amount of successful collisions that take place in a certain amount of time is increased as the temperature increases. We can also investigate to see if the 10ºC rise rule applies. This is that every 10ºC rise in temperature leads to the rate of reaction doubling. This should apply up to a point, but at a certain temperature the enzyme starts to denature so the reaction rate will then decrease. Using a pipette take ...

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