... certain conditions. It can be denatured in high phs and high temperatures. The bonds, which hold the enzyme together, are fairly weak, so they can be easily broken. Once the bonds of the enzyme break, the enzymes active site begins to change in a large way, this means that the enzyme is becoming deactivated. This is called denatured. Prediction: As the temperature of the solution increases, the amount of CO2 produced will increase; this is because the enzymes substrate will change, being more reactive. When the substrate changes too much, the cells active site will begin to de-nature, so the substrate is unable to work. This will bring down the amount of CO2 made. As the temperature of the enzymes hit its optimum working temperature, the molecules in the cells move around more, gaining a lot more energy. Method: We will set up the equipment as in the diagram; next we will heat a ...

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