... The boiling tube was placed in a water bath for 8 minutes. They were then removed and cooled. The colour was recorded. 1b 3cm³ 0.1% Glucose and 1cm³ Dilute Hydrochloric acid were pipetted into a boiling tube. The boiling tube was placed in the water bath until the solution had boiled for 2 minutes. The boiling tube was then removed and allowed to cool. The solution was then neutralised using Sodium Hydrogen Carbonate. Note: Enough of the Sodium Hydrogen Bicarbonate was used to stop the solution effervescing. Neutralisation was checked using Litmus paper. Another 5cm³ Benedict's reagent was added and the boiling tube was replaced in the water bath for 8 minutes. The boiling tube was then removed, allowed to cool and the colour was noted. 1c 3cm³ 0.1% Glucose solution and a few drops of 10% sucrase enzyme were added together. The boiling tube was then left for two ...

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